Recombinant Human FGF2 Protein, His-tagged

• Cat.No.: FGF2-1487H
• Product Overview: Recombinant Human FGF2 Protein (Pro143-Ser288) with N-His tag was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 20.1 kDa
  Accurate Molecular Mass: 20 kDa
• Protein length: Pro143-Ser288
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: FGF2 fibroblast growth factor 2 (basic) [ Homo sapiens (human) ]
• Official Symbol: FGF2
• Synonyms: FGF2; fibroblast growth factor 2 (basic); FGFB; fibroblast growth factor 2; prostatropin; heparin-binding growth factor 2; basic fibroblast growth factor bFGF; BFGF; FGF-2; HBGF-2
• Gene ID: 2247
• mRNA Refseq: NM_002006
• Protein Refseq: NP_001997
• MIM: 134920
• UniProt ID: P09038

Reviews

07/14/2022

The technical expert communicated with me in time to understand the progress of my experiment and gave me suggestions for improvement.

02/04/2022

The purchased protein is suitable for WB experiments and I am satisfied with the results.

Q&As

What is the biological function of FGF2?

FGF2 promotes cell migration and angiogenesis and regulates bone homeostatic balance.

Where is FGF2 mainly expressed?

FGF2 is widely expressed in the nervous system.

In which tissues of sheep is FGF2 primarily expressed?

FGF2 is mainly expressed in tissues such as mammary glands and ovaries of sheep.

What protein does FGF2 share high homology with?

FGF1 has a high degree of homology with FGF2.

What diseases is FGF2 involved in?

FGF2 is involved in diseases such as cardiovascular disease, breast disease and bone homeostasis.

How does FGF2 regulate bone homeostasis?

FGF2 negatively regulates bone homeostasis.