Recombinant Human FGG Protein (27-437aa), N-His tagged

• Cat.No.: FGG-01H
• Product Overview: Recombinant human FGG (27-437aa), fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Description: The protein encoded by this gene is the gamma component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia. Alternative splicing results in transcript variants encoding different isoforms.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Liquid
• Molecular Mass: 48.9 kDa (434aa) confirmed by MALDI-TOF
• Protein Length: 27-437aa
• AA Sequence: YVATRDNCCILDERFGSYCPTTCGI ADFLSTYQTKVDKDLQSLEDILHQV ENKTSEVKQLIKAIQLTYNPDESSK PNMIDAATLKSRKMLEEIMKYEASI LTHDSSIRYLQEIYNSNNQKIVNLK EKVAQLEAQCQEPCKDTVQIHDITG KDCQDIANKGAKQSGLYFIKPLKAN QQFLVYCEIDGSGNGWTVFQKRLDG SVDFKKNWIQYKEGFGHLSPTGTTE FWLGNEKIHLISTQSAIPYALRVEL EDWNGRTSTADYAMFKVGPEADKYR LTYAYFAGGDAGDAFDGFDFGDDPS DKFFTSHNGMQFSTWDNDNDKFEGN CAEQDGSGWWMNKCHAGHLNGVYYQ GGTYSKASTPNGYDNGIIWATWKTR WYSMKKTTMKIIPFNRLTIGEGQQH HLGGAKQAGDV
• Purity: > 90% by SDS-PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/mL (determined by Bradford assay)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Gene Information

• Gene Name: FGG fibrinogen gamma chain [ Homo sapiens (human) ]
• Official Symbol: FGG
• Synonyms: FGG; fibrinogen gamma chain; fibrinogen gamma chain; fibrinogen, gamma polypeptide; testicular tissue protein Li 70
• Gene ID: 2266
• mRNA Refseq: NM_000509
• Protein Refseq: NP_000500
• MIM: 134850
• UniProt ID: P02679