Recombinant Human Fibrillarin Protein, His-tagged

• Cat.No.: FBL-84H
• Product Overview: Recombinant Human Fibrillarin protein, fused with N-terminal His tag, was expressed in E. coli.

Specification

• Description: Human recombinant fibrillarin (residues 2-321; Genbank Accession # NM_001436; MW= 35.1 kDa) expressed in E. coli with an N-terminal His-tag. Fibrillarin is a component of nucleolar ribonucleoprotein complexes including snoRNPs (small nucleolar ribonucleoproteins) and Cajal bodies. As part of the C/D snoRNPs, fibrillarin functions as the catalytic subunit in the 2'-O-methylation of ribosomal RNAs1. In addition, fibrillarin has recently been shown to catalyze methylation of histone H2A glutamine-104, a modification restricted to the nucleolus and linked to RNA Pol I transcriptional activity on rDNA3. A distinct protein subcomplex comprising fibrillarin, SF2A-p32 and the argininine methyltransferases PRMT1 and PRMT5 has been described4 and fibrillarin's N-terminal glycine and arginine-rich (GAR) domain, including in the context of full-length fibrillarin5, is a substrate for various PRMTs.
• Source: E. coli
• Species: Human
• Tag: His
• Form: 50 mM Tris HCl, pH 7.5, 500 mM NaCl, 1 mM TCEP, 10 % glycerol
• Molecular Mass: 34.6 kDa
• Protein length: 1-321
• Purity: >85% by SDS-PAGE
• Notes: This product is NOT intended for therapeutic or diagnostic use in animals or in humans.
• Storage: Store at -70 centigrade. Thaw quickly and store on ice before use. The remaining, unused, undiluted protein should be snap frozen, for example in a dry/ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted protein is not recommended.

Gene Information

• Gene Name: FBL fibrillarin [ Homo sapiens ]
• Official Symbol: FBL
• Synonyms: FBL; fibrillarin; FIB; FLRN; RNU3IP1
• Gene ID: 2091
• mRNA Refseq: NM_001436
• Protein Refseq: NP_001427
• MIM: 134795
• UniProt ID: P22087