Recombinant Human FLG2 protein, His-tagged

• Cat.No.: FLG2-1604H
• Product Overview: Recombinant Human FLG2 aa. (Val1915~Arg2143 (Accession # Q5D862)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Val1915~Arg2143
• Description: The filaggrin-like protein encoded by this gene is upregulated by calcium, proteolyzed by calpain 1, and is involved in epithelial homeostasis. The encoded protein is required for proper cornification in skin, with defects in this gene being associated with skin diseases. This protein also has a function in skin barrier protection. In fact, in addition to providing a physical barrier, C-terminal fragments of this protein display antimicrobial activity against P. aeruginosa.
• Form: Freeze-dried powder
• Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.
• Isoelectric Point: 7.7

Gene Information

• Gene Name: FLG2 filaggrin family member 2 [ Homo sapiens (human) ]
• Official Symbol: FLG2
• Synonyms: FLG2; filaggrin family member 2; IFPS; filaggrin-2; ifapsoriasin; intermediate filament-associated and psoriasis susceptibility protein
• Gene ID: 388698
• mRNA Refseq: NM_001014342.2
• Protein Refseq: NP_001014364.1
• UniProt ID: Q5D862