Active Recombinant Human FN1 protein, His-tagged

• Cat.No.: FN1-2176H
• Product Overview: Active Recombinant Human FN1 protein(NP_997647.1)(Asn1722-Thr1811) is expressed in HEK293, fused with a 6xHis tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1722-1811 aa
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells.When cells are added to Fibronectin coated plates (2.5 μg/mL and 100 μL/well), approximately 30%-40% cells will adhere specifically after 30 minutes at 37°C.
• Molecular Mass: 10.70 kDa
• Endotoxin: < 1 EU/μg of the protein by LAL method.
• Purity: ≥ 90 % as determined by SDS-PAGE.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: FN1 fibronectin 1 [ Homo sapiens ]
• Official Symbol: FN1
• Synonyms: FN1; fibronectin 1; fibronectin; CIG; cold insoluble globulin; FINC; GFND2; LETS; migration stimulating factor; MSF; cold-insoluble globulin; migration-stimulating factor; FN; FNZ; ED-B; GFND; DKFZp686H0342; DKFZp686I1370; DKFZp686F10164; DKFZp686O13149
• Gene ID: 2335
• mRNA Refseq: NM_002026
• Protein Refseq: NP_002017
• MIM: 135600
• UniProt ID: P02751