Product overview : |
Recombinant Human FNDC5 (Q8NAU1) aa. (Asp32~Glu143) fused with His tag was produced in HEK293F cells. |
Description : |
This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants. |
Source : |
HEK293F |
Species : |
Human |
Tag : |
His |
Form : |
Freeze-dried powder |
Molecular Mass : |
Predicted Molecular Mass: 14.2kDa; Accurate Molecular Mass: 27kDa as determined by SDS-PAGE reducing conditions. |
Protein length : |
Asp32~Glu143 |
Endotoxin : |
<1.0EU per 1ug (determined by the LAL method) |
Purity : |
> 98% |
Characteristic : |
Isoelectric point is 5. |
Applications : |
Cell culture; Activity Assays; In vivo assays. |
Stability : |
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. |
Storage : |
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. |
Storage buffer : |
PBS, pH7.6, containing 5% trehalose. |
Reconstitution : |
Reconstitute in PBS (pH7.6) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |