Recombinant Human FOXO3 protein, His & T7-tagged

• Cat.No.: FOXO3-7633H
• Product Overview: Recombinant Human FOXO3 aa. (Leu369~Met633 (Accession # O43524)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed.
• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 32.0kDa
• Protein length: Leu369~Met633 (Accession # O43524)
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >90%
• Characteristic: The isoelectric point is 4.8.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.

Gene Information

• Gene Name: FOXO3 forkhead box O3 [ Homo sapiens (human) ]
• Official Symbol: FOXO3
• Synonyms: FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2; forkhead box O3A; forkhead homolog (rhabdomyosarcoma) like 1; forkhead in rhabdomyosarcoma-like 1; forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1; forkhead box protein O3
• Gene ID: 2309
• mRNA Refseq: NM_001455.3
• Protein Refseq: NP_001446.1
• UniProt ID: O43524