Recombinant Human FOXO3A protein, His-tagged

• Cat.No.: FOXO3A-2500H
• Product Overview: Recombinant Human FOXO3A protein(325-673 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 325-673 aa
• AA Sequence: SPIMASTELDEVQDDDAPLSPMLYS SSASLSPSVSKPCTVELPRLTDMAG TMNLNDGLTENLMDDLLDNITLPPS QPSPTGGLMQRSSSFPYTTKGSGLG SPTSSFNSTVFGPSSLNSLRQSPMQ TIQENKPATFSSMSHYGNQTLQDLL TSDSLSHSDVMMTQSDPLMSQASTA VSAQNSRRNVMLRNDPMMSFAAQPN QGSLVNQNLLHHQHQTQGALGGSRA LSNSVSNMGLSESSSLGSAKHQQQS PVSQSMQTLSDSLSGSSLYSTSANL PVMGHEKFPSDLDLDMFNGSLECDM ESIIRSELMDADGLDFNFDSLISTQ NVVGLNVGNFTGAKQASSQSWVPG
• Purity: 85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: FOXO3 forkhead box O3 [ Homo sapiens ]
• Official Symbol: FOXO3A
• Synonyms: FOXO3; forkhead box O3; FKHRL1, FOXO3A; forkhead box protein O3; AF6q21; FOXO2; forkhead box O3A; forkhead in rhabdomyosarcoma-like 1; forkhead homolog (rhabdomyosarcoma) like 1; forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1; FKHRL1; FOXO3A; FKHRL1P2; MGC12739; MGC31925; DKFZp781A0677
• Gene ID: 2309
• mRNA Refseq: NM_001455.3
• Protein Refseq: NP_001446.1
• MIM: 602681
• UniProt ID: O43524