| Species : |
Human |
| Source : |
InsectCells |
| Tag : |
His |
| Protein Length : |
1-594 aa |
| Description : |
Glutamate decarboxylase or glutamic acid decarboxylase (GAD) is an enzyme that catalyzes the decarboxylation of glutamate to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in higher brain regions, and putative paracrine hormone in pancreatic islets. GAD uses pyridoxal-phosphate (PLP) as a cofactor.Two molecular forms of GAD (65 kDa and 67 kDa) are highly conserved and both are expressed in the CNS, pancreatic islet cells, testis, oviduct and ovary. The isoforms are regionally distributed cytoplasmically in the brains of rats and mice. GAD65 is an ampiphilic, membrane-anchored protein (585 a.a.), and is responsible for vesicular GABA production. GAD67 is cytoplasmic (594 a.a.), and seems to be responsible for significant cytoplasmic GABA production. |
| Form : |
Supplied as 0.2 μm filtered solution in 20 mM Tris, 500 mM Nacl, pH8.5 with glycerol as protectant. |
| Bio-activity : |
Immobilized Human GAD1, His Tag at 1 μg/mL (100 μL/well) can bind Anti-GAD1 Antibody with a linear range of 1-8 ng/mL. |
| Molecular Mass : |
The protein has a calculated MW of 68.8 kDa. The protein migrates as 60-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Endotoxin : |
Less than 1.0 EU/μg by the LAL method/rFC method. |
| Purity : |
>85% as determined by SDS-PAGE. |
| Storage : |
This product is stable after storage at: The product MUST be stored at -70 centigrade or lower upon receipt; -70 centigrade for 3 months under sterile conditions. Please avoid repeated freeze-thaw cycles. |
| Concentration : |
0.2 mg/mL |
| Shipping : |
This product is supplied and shipped with dry ice, please inquire the shipping cost. |
