Recombinant Human Gamma-Glutamyl Hydrolase (conjugase, folylpolygammaglutamyl hydrolase), His-tagged

• Cat.No.: GGH-1399H
• Product Overview: Recombinanthuman GGHprotein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventionalchromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: GGH is a homodimericprotein that catalyzes the cleavage of the gamma-glutamyl chain offolylpoly-gamma-glutamyl substrates and is a central enzyme in folyl andantifolyl poly-gamma-glutamate metabolism. GGH may play an important role inthe bioavailability of dietary pteroylpolyglutamates and in the metabolism ofpteroylpolyglutamates and antifolates.
• Form: Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl.
• Molecular Weight: 35.9 kDa(315aa), confirmed by MALDI-TOF.
• Purity: > 95%by SDS-PAGE
• Concentration: 0.5mg/ml(determined by Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MRPHGDTAKK PIIGILMQKC RNKVMKNYGR YYIAASYVKY LESAGARVVPVRLDLTEKDY EILFKSINGI LFPGGSVDLR RSDYAKVAKI FYNLSIQSFDDGDYFPVWGT CLGFEELSLL ISGECLLTAT DTVDVAMPLN FTGGQLHSRM FQNFPTELLL SLAVEPLTANFHKWSLSVKN FTMNEKLKKF FNVLTTNTDG KIEFISTMEG YKYPVYGVQW HPEKAPYEWK NLDGISHAPNAVKTAFYLAEFFVNEARKNNHHFKSESEEE KALIYQFSPI YTGNISSFQQ CYIFD
• Storage: Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• OfficialSymbol: GGH
• Pathways: FluoropyrimidineActivity; Folate biosynthesis

Gene Information

• Gene Name: GGH gamma-glutamyl hydrolase(conjugase, folylpolygammaglutamyl hydrolase) [ Homo sapiens ]
• Synonyms: GGH; gamma-glutamyl hydrolase (conjugase,folylpolygammaglutamyl hydrolase); GH; gamma-glutamyl hydrolase; gamma-Glu-Xcarboxypeptidase; OTTHUMP00000226541; EC 3.4.19.9; Gamma-glutamyl hydrolase; Conjugase; Gamma-Glu-Xcarboxypeptidase; OTTHUMP00000226541; OTTHUMP00000226558
• Gene ID: 8836
• mRNA Refseq: NM_003878
• Protein Refseq: NP_003869
• MIM: 601509
• UniProt ID: Q92820
• Chromosome Location: 8q12.3