Recombinant Human GCAT Protein, GST-tagged

• Cat.No.: GCAT-4788H
• Product Overview: Human GCAT full-length ORF ( AAH14457, 1 a.a. - 419 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. This gene encodes the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The encoded enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase. [provided by RefSeq
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 71.61 kDa
• AA Sequence: MWPGNAWRAALFRVPRGRRAQSALA QLRGILEGELEGICGAGTWKSERVI TSRQGPHIRVDGVSGGILNFCANNY LGLSSHPEVIQAGLQALEEFGAGLS SVRFICGTQSIHKNLEAKIARFHQR EDAILYPSCYDANAGLFEALLTPED AVLSDELNHASIIDGIRLCKAHKYR YRHLDMADLEAKLQEAQKHRLRLVA TDGAFSMDGDIAPLQEICCLASRYG ALVFMDECHATGFLGPTGRGTDELL GVMDQVTIINSTLGKALGGASGGYT TGPGPLVSLLRQRARPYLFSNSLPP AVVGCASKALDLLMGSNTIVQSMAA KTQRFRSKMEAAGFTISGASHPICP VMLGDARLASRMADDMLKRGIFVIG FSYPVVPKGKAWIRVQISAVHSEED IDRCVEAFVEVGRLHGALP
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: GCAT glycine C-acetyltransferase [ Homo sapiens ]
• Official Symbol: GCAT
• Synonyms: GCAT; glycine C-acetyltransferase; glycine C acetyltransferase (2 amino 3 ketobutyrate coenzyme A ligase); 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial; 2 amino 3 ketobutyrate coenzyme A ligase; KBL; AKB ligase; aminoacetone synthase; glycine acetyltransferase; 2-amino-3-ketobutyrate-CoA ligase; MGC23053
• Gene ID: 23464
• mRNA Refseq: NM_001171690
• Protein Refseq: NP_001165161
• MIM: 607422
• UniProt ID: O75600