Recombinant Human GCKR Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: GCKR-6373H
• Product Overview: GCKR MS Standard C13 and N15-labeled recombinant protein (NP_001477) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a protein belonging to the GCKR subfamily of the SIS (Sugar ISomerase) family of proteins. The gene product is a regulatory protein that inhibits glucokinase in liver and pancreatic islet cells by binding non-covalently to form an inactive complex with the enzyme. This gene is considered a susceptibility gene candidate for a form of maturity-onset diabetes of the young (MODY).
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 69.1 kDa
• AA Sequence: MPGTKRFQHVIETPEPGKWELSGYE AAVPITEKSNPLTQDLDKADAENIV RLLGQCDAEIFQEEGQALSTYQRLY SGSILTTMVQVAGKVQEVLKEPDGG LVVLSGGGTSGRMAFLMSVSFNQLM KGLGQKPLYTYLIAGGDRSVVASRE GTEDSALHGIEELKKVAAGKKRVIV IGISVGLSAPFVAGQMDCCMNNTAV FLPVLVGFNPVSMARNDPIEDWSST FRQVAERMQKMQEKQKAFVLNPAIG PEGLSGSSRMKGGSATKILLETLLL AAHKTVDQGIAASQRCLLEILRTFE RAHQVTYSQSPKIATLMKSVSTSLE KKGHVYLVGWQTLGIIAIMDGVECI HTFGADFRDVRGFLIGDHSDMFNQK AELTNQGPQFTFSQEDFLTSILPSL TEIDTVVFIFTLDDNLTEVQTIVEQ VKEKTNHIQALAHSTVGQTLPIPLK KLFPSIISITWPLLFFEYEGNFIQK FQRELSTKWVLNTVSTGAHVLLGKI LQNHMLDLRISNSKLFWRALAMLQR FSGQSKARCIESLLRAIHFPQPLSD DIRAAPISCHVQVAHEKEQVIPIAL LSLLFRCSITEAQAHLAAAPSVCEA VRSALAGPGQKRTADPLEILEPDVQ TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: GCKR glucokinase regulator [ Homo sapiens (human) ]
• Official Symbol: GCKR
• Synonyms: GCKR; glucokinase (hexokinase 4) regulator; glucokinase (hexokinase 4) regulatory protein; glucokinase regulatory protein; GKRP; FGQTL5
• Gene ID: 2646
• mRNA Refseq: NM_001486
• Protein Refseq: NP_001477
• MIM: 600842
• UniProt ID: Q14397