Recombinant Human GCLC Protein, MYC/DDK-tagged

• Cat.No.: GCLC-668H
• Product Overview: Recombinant Human GCLC fused with MYC/DDK tag at C-terminal was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.
• Form: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 72.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >50 ug/mL as determined by microplate BCA method

Gene Information

• Gene Name: GCLC glutamate-cysteine ligase, catalytic subunit [ Homo sapiens ]
• Official Symbol: GCLC
• Synonyms: GCLC; glutamate-cysteine ligase, catalytic subunit; GLCL, GLCLC; glutamate--cysteine ligase catalytic subunit; GCS; gamma-ECS; GCS heavy chain; gamma-glutamylcysteine synthetase; GCL; GLCL; GLCLC
• Gene ID: 2729
• mRNA Refseq: NM_001498
• Protein Refseq: NP_001489
• MIM: 606857
• UniProt ID: P48506