Recombinant Human GDNF Family Receptor Alpha 3

• Cat.No.: GFRA3-1620H

Specification

• Species: Human
• Source: Sf21 Cells
• Tag: Non
• Description: GDNF family receptor alpha 3, also known as GFRA3 is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor and a member of the GDNF receptor family. It forms a signaling receptor complex with RET tyrosine kinase receptor and binds the ligand, artemin. GFRalpha3 preferentially binds the newest member of the glial-derived neurotrophic factor family of ligands, artemin. The major site of GFRalpha3 expression is in the dorsal root ganglion. GFRalpha3 artemin neurons are immunopositive for markers expected of nociceptors and include a subset of neurons distinct from the GDNF-responsive population.
• State Of Matter: Lyophilized.
• Purity: >90% by SDS Page and analyzed by silver stain.
• Endotoxin: <1.0 EU/µg as determined by the LAL method.
• Molecular Weight: The predicted molecular weight of Recombinant Human GFRα-3 is Mr 67 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 67 kDa.
• Storage And Stability: This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Gene Information

• Gene Name: GFRA3 GDNF family receptor alpha 3 [ Homo sapiens ]
• Synonyms: GDNF family receptor alpha 3; GFRA3; GPI-linked receptor; glial cell line-derived neurotrophic factor receptor alpha-3; GFR-alpha-3; GFRa-3
• Gene ID: 2676
• mRNA Refseq: NM_001496
• Protein Refseq: NP_001487
• MIM: 605710
• UniProt ID: O60609
• Chromosome Location: 5q31.1-q31.3
• Function: axon guidance receptor activity; receptor activity; receptor binding

Citation

Label-free epitope binning assays of monoclonal antibodies enable the identification of antigen heterogeneity.

Journal: Journal of immunological methods    PubMed ID: 22609372    Data: 2012/10/15

Authors: Yasmina N Abdiche, Kevin C Lindquist, Jaume Pons

Article Snippet: Purified recombinant hGFRα3 monomer expressed in human cells was purchased from Creative BioMart (Shirley, NY, Catalog# GFRA3-3153H) ; we refer to this preparation as P3. Purified recombinant hGFRα3 monomer expressed in human cells was purchased from Creative BioMart (Shirley, NY, Catalog# GFRA3-3153H) ; we refer to this preparation as P3.. Three preparations (referred to here as P4-Fc, P5-Fc, and P6-Fc) of purified recombinant hGFRα3 fused to a human-Fcγ1 were purchased commercially; P4-Fc (R&DSystems, Catalog# 670-FR) and P5-Fc (Creative BioMart, Catalog#GFRA3-1620H)were both expressed in insect cells, whereas preparation P6-Fc (Creative BioMart, Catalog#GFRQ3-3242H )was expressed in human cells.. Purified recombinant hFABP4 with a C-terminal 6-His tag was prepared in-house in mammalian cells.Purified recombinant hFABP4 with a C-terminal 6-His tag was prepared in-house in mammalian cells.