Recombinant Human GIF Protein (Met1-Tyr417), C-His tagged, Animal-free, Carrier-free

• Cat.No.: GIF-121H
• Product Overview: Recombinant Human GIF Protein (Met1-Tyr417) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Met1-Tyr417
• Description: Human Gastric intrinsic factor (GIF) is an intrinsic factor of the eukaryotic cobalamin family. Gastric intrinsic factor is a 44 kDa protein containing 380 amino acid residues. It is secreted from the parietal cells in gastric mucosa. Gastric intrinsic factor plays a major role in absorption and transportation of cobalamin and Cbl. It could form the comprised with Vit.B12 that avoid the destroy of Vit.B12.
• Form: Lyophilized
• Molecular Mass: The protein has a calculated MW of 46.23 kDa.
  The protein migrates as 45 kDa under reducing condition (SDS-PAGE analysis).
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: GIF gastric intrinsic factor (vitamin B synthesis) [ Homo sapiens (human) ]
• Official Symbol: GIF
• Synonyms: GIF; gastric intrinsic factor (vitamin B synthesis); gastric intrinsic factor; IF; IFMH; INF; TCN3; intrinsic factor
• Gene ID: 2694
• mRNA Refseq: NM_005142
• Protein Refseq: NP_005133
• MIM: 609342
• UniProt ID: P27352