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Recombinant Human GLS protein, His & T7-tagged

Cat.No. : GLS-1607H
Product Overview : Recombinant Human GLS aa. (Ile221~Leu533 (Accession # O94925)) fused with N-terminal His & T7 tag was produced in E. coli cells.
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  • Gene Information
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Description : This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants.
Source : E. coli
Species : Human
Tag : His & T7
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 38.1kDa
Protein length : Ile221~Leu533 (Accession # O94925)
Endotoxin : <1.0EU per 1ug (determined by the LAL method)
Purity : >90%
Applications : SDS-PAGE; WB; ELISA; IP.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Storage buffer : Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Reconstitution : Reconstitute in sterile PBS, pH7.2-pH7.4.
Isoelectric Point : 5.9
Gene Name : GLS glutaminase [ Homo sapiens (human) ]
Official Symbol : GLS
Synonyms : GLS; glutaminase; GAC; GAM; KGA; GLS1; AAD20; glutaminase kidney isoform, mitochondrial; K-glutaminase; L-glutamine amidohydrolase; glutaminase C; glutaminase, phosphate-activated
Gene ID : 2744
mRNA Refseq : NM_001256310.1
Protein Refseq : NP_001243239.1
UniProt ID : O94925

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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What is the main function of GLS in cellular metabolism? 02/14/2022

GLS catalyzes the conversion of glutamine to glutamate, playing a crucial role in amino acid metabolism.

What role does GLS play in neurotransmitter production? 10/19/2021

GLS is vital for neurotransmitter production, influencing glutamate and GABA levels.

How does GLS contribute to glutaminolysis? 09/04/2021

It facilitates glutaminolysis, enabling cells to utilize glutamine for energy and biosynthesis.

How is GLS activity implicated in cancer cell proliferation? 03/03/2021

In cancer, upregulated GLS supports rapid proliferation by fueling energy and biosynthetic pathways.

Are there therapeutic strategies targeting GLS in disease treatment? 02/27/2021

Therapeutic strategies targeting GLS aim to inhibit its activity, especially in glutamine-dependent cancers.

What is the relationship between GLS activity and cellular energy production? 06/20/2020

GLS activity contributes to cellular ATP production by providing intermediates for the TCA cycle.

In which tissues is GLS highly expressed? 03/30/2020

GLS is highly expressed in tissues with rapid cell division and high metabolic rates, such as cancer cells.

Customer Reviews (3)

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Reviews
11/23/2022

    Highly recommend for experiments.

    08/20/2021

      Worked well, good quality.

      05/29/2021

        Satisfied with product performance.

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