Species : |
Human |
Source : |
E.coli |
Tag : |
His |
Protein Length : |
1-214 a.a. |
Description : |
GLTPD1 mediates the intracellular transfer of ceramide-1-phosphate between organelle membranes and the cell membrane. This protein is required for normal structure of the Golgi stacks. GLTPD1 can bind phosphoceramides with a variety of aliphatic chains, but has a preference for lipids with saturated C16:0 or monounsaturated C18:1 aliphatic chains, and is inefficient with phosphoceramides containing lignoceryl (C24:0). It plays a role in the regulation of the cellular levels of ceramide-1-phosphate, and thereby contributes to the regulation of phospholipase PLA2G4A activity and the release of arachidonic acid. Recombinant human GLTPD1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
Form : |
Liquid. In Phosphate buffered saline (pH7.4) containing 50% glycerol, 1mM DTT |
Molecular Mass : |
26.8 kDa (237aa) confirmed by MALDI-TOF |
AA Sequence : |
MGSSHHHHHH SSGLVPRGSH MGSMDDSETG FNLKVVLVSF KQCLDEKEEV LLDPYIASWK GLVRFLNSLG TIFSFISKDV VSKLRIMERL RGGPQSEHYR SLQAMVAHEL SNRLVDLERR SHHPESGCRT VLRLHRALHW LQLFLEGLRT SPEDARTSAL CADSYNASLA AYHPWVVRRA VTVAFCTLPT REVFLEAMNV GPPEQAVQML GEALPFIQRV YNVSQKLYAE HSLLDLP |
Purity : |
>90 % by SDS - PAGE |
Storage : |
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
Concentration : |
0.25 mg/ml (determined by Bradford assay) |