Recombinant Human GNAO1 Protein, His-tagged

• Cat.No.: GNAO1-2208H
• Product Overview: Recombinant Human GNAO1 Protein (Gly2-Tyr354) with a N-His tag was expressed in E. coli.

Specification

• Description: The protein encoded by this gene represents the alpha subunit of the Go heterotrimeric G-protein signal-transducing complex. Defects in this gene are a cause of early-onset epileptic encephalopathy. Two transcript variants encoding different isoforms have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 43.7 kDa
  Accurate Molecular Mass: 43 kDa
• Protein length: Gly2-Tyr354
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 80%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: GNAO1 guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O [ Homo sapiens (human) ]
• Official Symbol: GNAO1
• Synonyms: GNAO1; guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O; guanine nucleotide-binding protein G(o) subunit alpha; G ALPHA o; guanine nucleotide binding protein, alpha activating polypeptide O; GNAO; G-ALPHA-o; DKFZp686O0962
• Gene ID: 2775
• mRNA Refseq: NM_020988
• Protein Refseq: NP_066268
• MIM: 139311
• UniProt ID: P09471