Recombinant Human GPA33 Protein, His-tagged, Alexa Fluor 488 conjugated

• Cat.No.: GPA33-244HAF488
• Product Overview: Alexa Fluor 488 conjugated recombinant human GPA33 (Ile22-Val235) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: Glycoprotein A33 (GPA33) is also known as Cell surface A33 antigen, is a single-pass type I membrane protein which is expressed in normal gastrointestinal epithelium and in 95% of colon cancers. GPA33 The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily, which contains. GPA33 may play a role in cell-cell recognition and signaling.
• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized
• Molecular Mass: The protein has a calculated MW of 24.5 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Ile22-Val235
• N-terminal Sequence Analysis: Ile 22
• Endotoxin: < 1.0 EU/ μg by the LAL method.
• Purity: > 96 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 488 via amines
  Excitation Wavelength: 488 nm
  Emission Wavelength: 515­-545 nm
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: GPA33
• Official Symbol: GPA33
• Synonyms: GPA33; glycoprotein A33 (transmembrane); cell surface A33 antigen; A33; transmembrane glycoprotein A33; MGC129986; MGC129987
• Gene ID: 10223
• mRNA Refseq: NM_005814
• Protein Refseq: NP_005805
• MIM: 602171
• UniProt ID: Q99795

Reviews

12/03/2022

We valued the product's safety profile, which ensured the well-being of our researchers and the integrity of our experiments.

06/05/2019

The product's biocompatibility with various cell types and organisms made it versatile for a wide range of applications.

08/27/2018

The product's unique attributes propelled our research to innovative heights.

Q&As

Are there any known diseases associated with GPA33?

Colorectal cancer is associated with abnormal expression of GPA33.

Is GPA33 membrane-bound?

Yes, GPA33 is a membrane-associated protein.

What role does GPA33 play in cancer?

GPA33 is implicated in colorectal cancer and is used as a marker for cancer diagnosis.

Is there a known molecular function for GPA33?

The provided information doesn't specify a detailed molecular function for GPA33.

What is the significance of GPA33 in cell adhesion?

GPA33's role in cell adhesion suggests it may have implications in cellular interactions and tissue organization.

What is the function of GPA33?

GPA33 is a cell surface protein involved in cell adhesion and is associated with colorectal cancer.

Is GPA33 widely expressed in tissues?

The provided information doesn't specify the tissue expression pattern of GPA33.