Recombinant Human GPX4 Protein, His-tagged GPX4-01H

Recombinant Human GPX4 Protein, His-tagged

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Recombinant Human GPX4 Protein, His-tagged

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Cat.No. : GPX4-01H
Product Overview : Recombinant human GPX4 protein (1-170) with a N-terminal His-tag was expressed in E. coli strain C321.ΔA.exp.
Description : The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Mutations in this gene are associated with Sedaghatian type of spondylometaphyseal dysplasia (SMDS). This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Transcript variants resulting from alternative splicing or use of alternate promoters have been described to encode isoforms with different subcellular localization.
Source : E. coli
Species : Human
Tag : His
Form : 50 mM potassium phosphate buffer, pH 7.6, with 0.1 mM DTT and 5% glycerol
Molecular Mass : 21.2 kDa
Protein length : 1-170
Purity : ≥65% estimated by SDS-PAGE
Unit Definition : One unit is defined as the amount of enzyme required to produce 1 µmol of NADP+ per minute at 25 centigrade in 50 mM Tris-HCl, pH 7.6, with 5 mM EDTA, 1 mM GSH, 0.076 units glutathione reductase, and 263 µM NADPH
Applications : Enzyme Activity, WB, ELISA
Stability : ≥ 1 year
Storage : Store at -80 centigrade
SDS-PAGE Analysis of GPX4 :

Lane 1: MW Markers, Lane 2: GPX4 (2 µg), Lane 3: GPX4 (4 µg), SDS-PAGE analysis of GPX4. Representative gel image shown; actual purity may vary between each batch.

Figure :

GPX4 activity was determined using our Glutathione Peroxidase Assay Kit.

Gene Name : GPX4 glutathione peroxidase 4 [ Homo sapiens (human) ]
Official Symbol : GPX4
Synonyms : GPX4; glutathione peroxidase 4; MCSP; SMDS; GPx-4; PHGPx; snGPx; GSHPx-4; snPHGPx; phospholipid hydroperoxide glutathione peroxidase; epididymis secretory sperm binding protein; phospholipid hydroperoxidase; phospholipid hydroperoxide glutathione peroxidase, mitochondrial; sperm nucleus glutathione peroxidase
Gene ID : 2879
mRNA Refseq : NM_001039847
Protein Refseq : NP_001034936
MIM : 138322
UniProt ID : P36969

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