Recombinant Human GTF2E1 293 Cell Lysate

• Cat.No.: GTF2E1-5702HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for general transcription factor IIE, polypeptide 1, alpha 56kDa (GTF2E1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: GTF2E1 general transcription factor IIE, polypeptide 1, alpha 56kDa [ Homo sapiens ]
• Official Symbol: GTF2E1
• Synonyms: GTF2E1; general transcription factor IIE, polypeptide 1, alpha 56kDa; general transcription factor IIE, polypeptide 1 (alpha subunit, 56kD); general transcription factor IIE subunit 1; FE; TFIIE A; TFIIE-alpha; general transcription factor IIE 56 kDa subunit; transcription initiation factor IIE subunit alpha; TF2E1; TFIIE-A
• Gene ID: 2960
• mRNA Refseq: NM_005513
• Protein Refseq: NP_005504
• MIM: 189962
• UniProt ID: P29083
• Chromosome Location: 3q21-q24
• Pathway: Basal transcription factors, organism-specific biosystem; Basal transcription factors, conserved biosystem; Disease, organism-specific biosystem; Eukaryotic Transcription Initiation, organism-specific biosystem; Gene Expression, organism-specific biosystem; HIV Infection, organism-specific biosystem; HIV Life Cycle, organism-specific biosystem
• Function: metal ion binding; protein binding; sequence-specific DNA binding; zinc ion binding