Recombinant Human GUSB Protein, GST-tagged

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Recombinant Human GUSB Protein, GST-tagged

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Cat.No. : GUSB-4500H
Product Overview : Human GUSB full-length ORF ( AAH14142.1, 22 a.a. - 651 a.a.) recombinant protein with GST-tag at N-terminal.
Description : The GUSB gene encodes beta-glucuronidase (EC 3.2.1.31), a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans (Shipley et al., 1993 [PubMed 7680524]). It is a tetrameric glycoprotein composed of identical subunits (Oshima et al., 1987 [PubMed 3468507]). The GUSB gene is mutated in mucopolysaccharidosis type VII (MPS7; MIM 253220).[supplied by OMIM
Source : Wheat Germ
Species : Human
Tag : GST
Molecular Mass : 94.82 kDa
AA Sequence : GLQGGMLYPQ ESPSRECKEL DGLWSFRADF SDNRRRGFEE QWYRRPLWES GPTVDMPVPS SFNDISQDWR LRHFVGWVWY EREVILPERW TQDLRTRVVL RIGSAHSYAI VWVNGVDTLE HEGGYLPFEA DISNLVQVGP LPSRLRITIA INNTLTPTTL PPGTIQYLTD TSKYPKGYFV QNTYFDFFNY AGLQRSVLLY TTPTTYIDDI TVTTSVEQDS GLVNYQISVK GSNLFKLEVR LLDAENKVVA NGTGTQGQLK VPGVSLWWPY LMHERPAYLY SLEVQLTAQT SLGPVSDFYT LPVGIRTVAV TKSQFLINGK PFYFHGVNKH EDADIRGKGF DWPLLVKDFN LLRWLGANAF RTSHYPYAEE VMQMCDRYGI VVIDECPGVG LALPQFFNNV SLHHHMQVME EVVRRDKNHP AVVMWSVANE PASHLESAGY YLKMVIAHTK SLDPSRPVTF VSNSNYAADK GAPYVDVICL NSYYSWYHDY GHLELIQLQL ATQFENWYKK YQKPIIQSEY GAETIAGFHQ DPPLMFTEEY QKSLLEQYHL GLDQKRRKYV VGELIWNFAD FMTEQSPTRV LGNKKGIFTR QRQPKSAAFL LRERYWKIAN ETRYPHSVAK SQCLENSLFT
Applications : Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
Notes : Best use within three months from the date of receipt of this protein.
Storage : Store at -8 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Quality Control Testing :

12.5% SDS-PAGE Stained with Coomassie Blue.

Gene Name : GUSB glucuronidase, beta [ Homo sapiens ]
Official Symbol : GUSB
Synonyms : GUSB; glucuronidase, beta; beta-glucuronidase; beta-G1; beta-D-glucuronidase; BG; MPS7; FLJ39445;
Gene ID : 2990
mRNA Refseq : NM_000181
Protein Refseq : NP_000172
MIM : 611499
UniProt ID : P08236

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