Recombinant Human GUSB protein, His & T7-tagged

• Cat.No.: GUSB-6928H
• Product Overview: Recombinant Human GUSB aa. (Glu451~Leu649 (Accession # P08236)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Glu451~Leu649
• Description: This gene encodes a hydrolase that degrades glycosaminoglycans, including heparan sulfate, dermatan sulfate, and chondroitin-4,6-sulfate. The enzyme forms a homotetramer that is localized to the lysosome. Mutations in this gene result in mucopolysaccharidosis type VII. Alternative splicing results in multiple transcript variants. There are many pseudogenes of this locus in the human genome.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 27.1kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 8.7.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: GUSB glucuronidase beta [ Homo sapiens (human) ]
• Official Symbol: GUSB
• Synonyms: GUSB; glucuronidase beta; BG; MPS7; beta-glucuronidase; beta-D-glucuronidase; beta-G1
• Gene ID: 2990
• mRNA Refseq: NM_000181.3
• Protein Refseq: NP_000172.2
• UniProt ID: P08236