Recombinant Human H1F0 protein, His-tagged

• Cat.No.: H1F0-6945H
• Product Overview: Recombinant Human H1F0 protein(P07305)(Met1-Lys194), fused with His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized from sterile 50mM Tris, 600mM NaCl, 1mM DTT, pH 8.5Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human H1F0 consisting of 205 amino acids and has a calculated molecular mass of 22.4 kDa. It migrates as an approximately 27 kDa band in SDS-PAGE under reducing conditions.
• Protein length: Met1-Lys194
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: H1F0 H1 histone family, member 0 [ Homo sapiens ]
• Official Symbol: H1F0
• Synonyms: H1F0; H1 histone family, member 0; H1FV; histone H1.0; H1.0; H1(0); H1 0; H10; histone H1; histone H1(0); H1.0, H1(0), H1-0; MGC5241
• Gene ID: 3005
• mRNA Refseq: NM_005318
• Protein Refseq: NP_005309
• MIM: 142708
• UniProt ID: P07305

Reviews

09/13/2023

H1F0 has good stability and long-term storage without problems.

06/08/2023

Strong stability, can form a stable micelle structure.

11/18/2021

It performs well in western blotting experiments.

Q&As

Is there a relationship between the expression level of H1F0 and the disease?

Studies have shown that the expression level of H1F0 is associated with a variety of diseases. For example, in some cancers, the expression level of H1F0 is upregulated, which promotes the proliferation and metastasis of tumor cells. In some neurodegenerative diseases, the expression of H1F0 may be down-regulated, leading to cell death.

What is the relationship of H1F0 to other genes or proteins?

H1F0 has a complex relationship with other genes or proteins. For example, it interacts with some transcription factors and regulatory proteins of signal transduction pathways, affecting gene expression and cell signal transduction. In addition, H1F0 is also affected by some epigenetic modifications, such as methylation and acetylation.

How can we better understand the role of H1F0 in disease?

In order to better understand the role of H1F0 in diseases, further in-depth research is needed on its specific mechanism of action and function. In addition, it is also necessary to study the changes in its expression level and activity in different diseases, as well as its relationship with the occurrence and development of diseases. At the same time, it is also necessary to explore the clinical application prospects and effects of the therapeutic strategies that regulate them.

What is the location and role of H1F0 in cells?

H1F0 is mainly located in the nucleus, binds to chromatin (DNA-protein complex) and regulates its structure. It is able to influence the way DNA is folded and packaged, which in turn affects gene expression and DNA repair. In addition, H1F0 is also involved in the regulation of cell division and cell cycle.

What are the limitations of research on H1F0?

There are still some limitations in the current research on H1F0, such as the lack of in-depth research on its specific mechanism and function, and the incomplete understanding of its relationship with diseases. In addition, since H1F0 is a multifunctional protein, regulating it may also have some side effects.

Is there a treatment strategy for H1F0?

Therapeutic strategies for H1F0 include inhibiting its expression, modulating its activity, or using gene therapy. For example, some studies have shown that the use of small molecule inhibitors targeting H1F0 can inhibit its activity and thus treat some H1F0-related diseases. In addition, related diseases can also be treated by regulating the expression level of H1F0.