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Recombinant Human HARS protein, His-tagged

Cat.No. : HARS-8209H
Product Overview : Recombinant Human HARS aa. (Met1~Gln294) fused with N-terminal His tag was produced in E. coli cells.
  • Specification
  • Gene Information
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Description : Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Several transcript variants encoding different isoforms have been found for this gene.
Source : E. coli
Species : Human
Tag : His
Form : Freeze-dried powder
Molecular Mass : 37kDa as determined by SDS-PAGE reducing conditions.
Protein length : Met1~Gln294
Endotoxin : <1.0EU per 1ug (determined by the LAL method)
Purity : >93%
Characteristic : The isoelectric point is 8.1.
Applications : SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
Stability : The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Concentration : 200μg/mL
Storage Buffer : 100mM NaHCO3, 500mM NaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% Trehalose and Proclin300.
Reconstitution : Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Gene Name : HARS histidyl-tRNA synthetase [ Homo sapiens (human) ]
Official Symbol : HARS
Synonyms : HARS; histidyl-tRNA synthetase; HRS; CMT2W; USH3B; histidine--tRNA ligase, cytoplasmic; HisRS; histidine translase
Gene ID : 3035
mRNA Refseq : NM_001258040.2
Protein Refseq : NP_001244969.1
UniProt ID : P12081

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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