Recombinant Human Heat Shock 70kDa Protein 8, His-tagged

• Cat.No.: HSPA8-4712H
• Product Overview: Recombinant Human HSC70 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 666 amino acids (1-646 a.a.) and having a molecular mass of 73.1kDa. HSC70 human recombinant is fused to 20 amino acid His Tag at N-terminus and purified by convential chromatogrpahy techniques.

Specification

• Species: Human
• Source: Human
• Tag: His
• Protein Length: 1-646 a.a.
• Description: HSPA8 is part of the heat shock protein 70 family which includes both heat-inducible and constitutively expressed proteins (heat-shock cognate proteins). HSPA8 is a heat-shock cognate protein that binds to nascent polypeptides to facilitate correct folding. HSPA8 is directly linked with Influenza virus matrix protein 1 and thus required for viral production.
• Form: The HSC70 protein solution contains 20mM Tris, pH-8 & 10% Glycerol.
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Physical Appearance: Sterile filtered colorless solution.
• Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MSKGPAVGID LGTTYSCVGV FQHGKVEIIA NDQGNRTTPS YVAFTDTERL IGDAAKNQVA MNPTNTVFDA KRLIGRRFDD AVVQSDMKHW PFMVVNDAGR PKVQVEYKGE TKSFYPEEVS SMVLTKMKEI AEAYLGKTVT NAVVTVPAYF NDSQRQATKD AGTIAGLNVL RIINEPTAAA IAYGLDKKVG AERNVLIFDL GGGTFDVSIL TIEDGIFEVK STAGDTHLGG EDFDNRMVNH FIAEFKRKHK KDISENKRAV RRLRTACERA KRTLSSSTQA SIEIDSLYEG IDFYTSITRA RFEELNADLF RGTLDPVEKA LRDAKLDKSQ IHDIVLVGGS TRIPKIQKLL QDFFNGKELN KSINPDEAVA YGAAVQAAIL SGDKSENVQD LLLLDVTPLS LGIETAGGVM TVLIKRNTTI PTKQTQTFTT YSDNQPGVLI QVYEGERAMT KDNNLLGKFE LTGIPPAPRG VPQIEVTFDI DANGILNVSA VDKSTGKENK ITITNDKGRL SKEDIERMVQ EAEKYKAEDE KQRDKVSSKN SLESYAFNMK ATVEDEKLQG KINDEDKQKI LDKCNEIINW LDKNQTAEKE EFEHQQKELE KVCNPIITKL YQSAGGMPGG MPGGFPGGGA PPSGGASSGP TIEEVD.
• Storage: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
• Pathways: Antigen processing and presentation; C-MYB transcription factor network; Clathrin derived vesicle budding; Lysosome Vesicle Biogenesis; MAPK signaling pathway; Measles; Metabolism of RNA; Spliceosome; Toxoplasmosis

Gene Information

• Gene Name: HSPA8 heat shock 70kDa protein 8 [ Homo sapiens ]
• Official Symbol: HSPA8
• Synonyms: HSPA8; heat shock 70kDa protein 8; LAP1; HSC54; HSC70; HSC71; HSP71; HSP73; NIP71; HSPA10; MGC29929; MGC131511; LPS-associated protein 1; heat shock 70kD protein 8; heat shock 70kd protein 10; heat shock cognate protein 54; constitutive heat shock protein 70; heat shock cognate protein, 71-kDa; lipopolysaccharide-associated protein 1; N-myristoyltransferase inhibitor protein 71; Heat shock cognate 71 kDa protein; constitutive heat shock protein 70; heat shock 70kd protein 10
• Gene ID: 3312
• mRNA Refseq: NM_006597
• Protein Refseq: NP_006588
• MIM: 600816
• UniProt ID: P11142
• Chromosome Location: 11q24.1
• Function: ATP binding; ATPase activity, coupled; nucleotide binding; unfolded protein binding