Species : |
Human |
Source : |
E.coli |
Tag : |
His |
Protein Length : |
1-154 a.a. |
Description : |
Hematological and neurological expressed 1 protein, also known as HN1, is a small protein that is highly conserved among species. HN1 expression is upregulated in regenerating neural tissues, including the axotomized adult rodent facial motor nerve and dedifferentiating retinal pigment epithelial cells of the Japanese newt. It is also expressed in numerous tissues during embryonic development as well as in regions of the adult brain that exhibit high plasticity. Recombinant human HN1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
Form : |
Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol |
Molecular Mass : |
18.1kDa (174aa) confirmed by MALDI-TOF(Molecular size on SDS-PAGE will appear higher) |
AA Sequence : |
MGSSHHHHHH SSGLVPRGSH MTTTTTFKGV DPNSRNSSRV LRPPGGGSNF SLGFDEPTEQ PVRKNKMASN IFGTPEENQA SWAKSAGAKS SGGREDLESS GLQRRNSSEA SSGDFLDLKG EGDIHENVDT DLPGSLGQSE EKPVPAAPVP SPVAPAPVPS RRNPPGGKSS LVLG |
Purity : |
>90% by SDS - PAGE |
Storage : |
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
Concentration : |
0.5 mg/ml (determined by BCA assay) |