Recombinant Human HHLA2 protein, Fc-tagged

• Cat.No.: HHLA2-548H
• Product Overview: Recombinant Human HHLA2 (Ile23-Asn344) protein was fused to human IgG1 Fc tag tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: B7-H7 (HHLA2) is a newly identified B7 family member that regulates human T-cell functions. B7-H7 was previously known as human endogenous retrovirus-H long terminal repeat associating 2 (HHLA2) with unidentified function. Recently, B7-H7 has been identified as a specific ligand for human CD28H. The B7-H7-CD28H pathway strongly promoted CD4+ T-cell proliferation and cytokine production via an AKT-dependent signaling cascade in the presence of TCR signaling, suggesting B7-H7 comprises a new co-stimulatory pathway. The first IgV domain of B7-H7, which presumably binds to a putative receptor, shows the highest homology to other B7 family members.
• Source: HEK293
• Species: Human
• Tag: Fc
• Predicted N Terminal: Ile23
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 63.4 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Ile23-Asn344
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by reduced SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: HHLA2
• Official Symbol: HHLA2
• Synonyms: HHLA2; HERV-H LTR-associating 2; HERV-H LTR-associating protein 2; human endogenous retrovirus-H long terminal repeat-associating protein 2
• Gene ID: 11148
• mRNA Refseq: NM_007072
• Protein Refseq: NP_009003
• MIM: 604371
• UniProt ID: Q9UM44