Recombinant Human HLA-G protein, His/SUMO-tagged HLA-G-39H

Recombinant Human HLA-G protein, His/SUMO-tagged

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Recombinant Human HLA-G protein, His/SUMO-tagged

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Cat.No. : HLA-G-39H
Product Overview : Recombinant Human HLA-G protein (25-338aa) was fused to 6xHis/SUMO-tag at the N-terminus and expressed in E.coli.
Description : HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail.
Source : E.coli
Species : Human
Tag : His/SUMO
Form : Liquid or Lyophilized powder. If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Molecular Mass : 51.6 kDa
Protein length : 314
AA Sequence : GSHSMRYFSAAVSRP GRGEPRFIAMGYVDD TQFVRFDSDSACPRM EPRAPWVEQEGPEYW EEETRNTKAHAQTDR MNLQTLRGYYNQSEA SSHTLQWMIGCDLGS DGRLLRGYEQYAYDG KDYLALNEDLRSWTA ADTAAQISKRKCEAA NVAEQRRAYLEGTCV EWLHRYLENGKEMLQ RADPPKTHVTHHPVF DYEATLRCWALGFYP AEIILTWQRDGEDQT QDVELVETRPAGDGT FQKWAAVVVPSGEEQ RYTCHVQHEGLPEPL MLRWKQSSLPTIPIM GIVAGLVVLAAVVTG AAVAAVLWRKKSSD
Endotoxin : <1.0 EU per 1µg (determined by the LAL method).
Purity : Greater than 90% as determined by SDS-PAGE.
Storage : Store at -20 centigrade upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Expiry : The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20/-80 centigrade. The shelf life of lyophilized form is 12 months at -20/-80 centigrade.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20/-80 centigrade. Our default final concentration of glycerol is 50%. Customers could use it as reference.
SDS-PAGE of HLA-G-39H :

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Gene Name : HLA-G
Official Symbol : HLA-G
Synonyms : MHC-G
Gene ID : 3135
mRNA Refseq : NM_001363567.1
Protein Refseq : NP_001350496.1
MIM : 142871
UniProt ID : P17693

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