Recombinant Human HLCS Protein, GST-tagged

• Cat.No.: HLCS-4850H
• Product Overview: Human HLCS full-length ORF ( NP_000402.3, 1 a.a. - 726 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: Holocarboxylase synthetase (EC 6.3.4.10) covalently links biotin to propionyl-CoA carboxylase (PCCA; MIM 232000), pyruvate carboxylase (PC; MIM 608786), alpha-methylcrotonyl-CoA carboxylase (MCCC1; MIM 609010), and acetyl-CoA carboxylase (ACACA; MIM 200350).[supplied by OMIM
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 107.2 kDa
• AA Sequence: MEDRLHMDNGLVPQKIVSVHLQDST LKEVKDQVSNKQAQILEPKPEPSLE IKPEQDGMEHVGRDDPKALGEEPKQ RRGSASGSEPAGDSDRGGGPVEHYH LHLSSCHECLELENSTIESVKFASA ENIPDLPYDYSSSLESVADETSPER EGRRVNLTGKAPNILLYVGSDSQEA LGRFHEVRSVLADCVDIDSYILYHL LEDSALRDPWTDNCLLLVIATRESI PEDLYQKFMAYLSQGGKVLGLSSSF TFGGFQVTSKGALHKTVQNLVFSKA DQSEVKLSVLSSGCRYQEGPVRLSP GRLQGHLENEDKDRMIVHVPFGTRG GEAVLCQVHLELPPSSNIVQTPEDF NLLKSSNFRRYEVLREILTTLGLSC DMKQVPALTPLYLLSAAEEIRDPLM QWLGKHVDSEGEIKSGQLSLRFVSS YVSEVEITPSCIPVVTNMEAFSSEH FNLEIYRQNLQTKQLGKVILFAEVT PTTMRLLDGLMFQTPQEMGLIVIAA RQTEGKGRGGNVWLSPVGCALSTLL ISIPLRSQLGQRIPFVQHLMSVAVV EAVRSIPEYQDINLRVKWPNDIYYS DLMKIGGVLVNSTLMGETFYILIGC GFNVTNSNPTICINDLITEYNKQHK AELKPLRADYLIARVVTVLEKLIKE FQDKGPNSVLPLYYRYWVHSGQQVH LGSAEGPKVSIVGLDDSGFLQVHQE GGEVVTVHPDGNSFDMLRNLILPKR R
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -8 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: HLCS holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) [ Homo sapiens ]
• Official Symbol: HLCS
• Synonyms: HLCS; holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase); holocarboxylase synthetase (biotin (proprionyl Coenzyme A carboxylase (ATP hydrolysing)) ligase) , holocarboxylase synthetase (biotin [proprionyl Coenzyme A carboxylase (ATP hydrolysing)] ligase); biotin--protein ligase; HCS; biotin apo-protein ligase; biotin--[acetyl-CoA-carboxylase] ligase; biotin--[methylcrotonoyl-CoA-carboxylase] ligase; biotin--[methylmalonyl-CoA-carboxytransferase] ligase; holocarboxylase synthetase (biotin-(proprionyl-Coenzyme A-carboxylase (ATP-hydrolysing)) ligase)
• Gene ID: 3141
• mRNA Refseq: NM_000411
• Protein Refseq: NP_000402
• MIM: 609018
• UniProt ID: P50747