Recombinant Human HMGCL Protein, His-tagged

• Cat.No.: HMGCL-211H
• Product Overview: Recombinant Human HMGCL(28-325aa) fused with His tag at C-terminal was expressed in Insect cells.

Specification

• Description: HMGCL, also known as hydroxymethylglutaryl-CoA lyase, mitochondrial isoform 1, is a mitochondrial matrix protein that belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes the final step of leucine degradation and plays a key role in ketone body formation. Multiple isoforms of the proteins are known due to alternative splicing. The major isoform (isoform 1) is most highly expressed in the liver whereas isoform 2 is found in energy-demanding tissues including the brain, heart, and skeletal muscle.
• Source: Insect cells
• Species: Human
• Tag: His
• Form: Liquid. In Phosphate Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT.
• Molecular Mass: 32.5kDa (305aa) 28-40KDa (SDS-PAGE under reducing conditions.)
• Protein length: 28-325aa
• AA Sequence: MTLPKRVKIV EVGPRDGLQN EKNIVSTPVK IKLIDMLSEA GLSVIETTSF VSPKWVPQMG DHTEVLKGIQ KFPGINYPVL TPNLKGFEAA
  VAAGAKEVVI FGAASELFTK KNINCSIEES FQRFDAILKA AQSANISVRG YVSCALGCPY EGKISPAKVA EVTKKFYSMG CYEISLGDTI
  GVGTPGIMKD MLSAVMQEVP LAALAVHCHD TYGQALANTL MALQMGVSVV DSSVAGLGGC PYAQGASGNL ATEDLVYMLE GLGIHTGVNL
  QKLLEAGNFI CQALNRKTSS KVAQATCKLH HHHHH
• Purity: > 90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: HMGCL 3-hydroxymethyl-3-methylglutaryl-CoA lyase [ Homo sapiens ]
• Official Symbol: HMGCL
• Synonyms: HMGCL; 3-hydroxymethyl-3-methylglutaryl-CoA lyase; 3 hydroxymethyl 3 methylglutaryl Coenzyme A lyase; hydroxymethylglutaryl-CoA lyase, mitochondrial; HL; hydroxymethylglutaricaciduria; HMG-CoA lyase; 3-hydroxy-3-methylglutaryl-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase; 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase; mitochondrial 3-hydroxy-3-methylglutaryl-CoA lyase
• Gene ID: 3155
• mRNA Refseq: NM_000191
• Protein Refseq: NP_000182
• MIM: 613898
• UniProt ID: P35914