Recombinant Human HMGCL Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: HMGCL-4287H
• Product Overview: HMGCL MS Standard C13 and N15-labeled recombinant protein (NP_000182) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: The protein encoded by this gene belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes the final step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associated with HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Molecular Mass: 34.4 kDa
• AA Sequence: MAAMRKALPRRLVGLASLRAVSTSSMGTLPKRVKIVEVGPRDGLQNEKNIVSTPVKIKLIDMLSEAGLSVIETTSFVSPKWVPQMGDHTEVLKGIQKFPGINYPVLTPNLKGFEAAVAAGAKEVVIFGAASELFTKKNINCSIEESFQRFDAILKAAQSANISVRGYVSCALGCPYEGKISPAKVAEVTKKFYSMGCYEISLGDTIGVGTPGIMKDMLSAVMQEVPLAALAVHCHDTYGQALANTLMALQMGVSVVDSSVAGLGGCPYAQGASGNLATEDLVYMLEGLGIHTGVNLQKLLEAGNFICQALNRKTSSKVAQATCKLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: HMGCL 3-hydroxymethyl-3-methylglutaryl-CoA lyase [ Homo sapiens (human) ]
• Official Symbol: HMGCL
• Synonyms: HMGCL; 3-hydroxymethyl-3-methylglutaryl-CoA lyase; 3 hydroxymethyl 3 methylglutaryl Coenzyme A lyase; hydroxymethylglutaryl-CoA lyase, mitochondrial; HL; hydroxymethylglutaricaciduria; HMG-CoA lyase; 3-hydroxy-3-methylglutaryl-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase; 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase; mitochondrial 3-hydroxy-3-methylglutaryl-CoA lyase
• Gene ID: 3155
• mRNA Refseq: NM_000191
• Protein Refseq: NP_000182
• MIM: 613898
• UniProt ID: P35914