Recombinant Human HNF1A Protein, His-tagged

• Cat.No.: HNF1A-2249H
• Product Overview: Recombinant Human HNF1A Protein (Glu71-Arg271) with a N-His tag was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is a transcription factor required for the expression of several liver-specific genes. The encoded protein functions as a homodimer and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Defects in this gene are a cause of maturity onset diabetes of the young type 3 (MODY3) and also can result in the appearance of hepatic adenomas. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 26.1 kDa
  Accurate Molecular Mass: 34 kDa
• Protein length: Glu71-Arg271
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: HNF1A HNF1 homeobox A [ Homo sapiens (human) ]
• Official Symbol: HNF1A
• Synonyms: HNF1A; HNF1 homeobox A; MODY3, TCF1, transcription factor 1, hepatic; LF B1, hepatic nuclear factor (HNF1), albumin proximal factor; hepatocyte nuclear factor 1-alpha; HNF1; LFB1; HNF-1-alpha; albumin proximal factor; hepatic nuclear factor 1; transcription factor 1, hepatic; interferon production regulator factor; liver-specific transcription factor LF-B1; TCF1; MODY3; TCF-1; HNF-1A; IDDM20
• Gene ID: 6927
• mRNA Refseq: NM_000545
• Protein Refseq: NP_000536
• MIM: 142410
• UniProt ID: P20823