| Cat.No. : |
ICOS-378H
Optional Service: Optional requirements on this protein
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| Product Overview : |
Recombinant Human ICOS protein (C136S, C137S, Glu21-Phe141), fused to His tag at C-terminus, was expressed in human 293 cells (HEK293). |
| Description : |
Inducible T-cell costimulator (ICOS) is also known as Activation-inducible lymphocyte immunomediatory molecule (AILIM), CD278, which belongs to the CD28 family of immune costimulatory receptors consisting of CD28, CTLA-4 and PD-1. ICOS enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. CD278/ICOS prevents the apoptosis of pre-activated T-cells and also plays a critical role in CD40-mediated class switching of immunoglobin isotypes. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
His |
| Form : |
Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose. |
| Molecular Mass : |
The protein has a calculated MW of 15.2 kDa. The protein migrates as 23-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Protein length : |
121 |
| Endotoxin : |
Less than 1.0 EU per ug by the LAL method. |
| Purity : |
>85% as determined by SDS-PAGE. |
| Storage : |
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents. |
| SDS-PAGE of ICOS-378H : |
Human ICOS (C136S, C137S), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.
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| Bioactivity-ELISA of ICOS-378H : |
Immobilized Human B7-H2, Fc Tag at 1 μg/mL (100 μL/well) can bind Human ICOS (C136S, C137S), His Tag with a linear range of 0.008-0.125 μg/mL (QC tested).
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.