| Description : |
This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
117.8 kDa |
| AA Sequence : |
MRYRLAWLLHPALPSTFRSVLGARL PPPERLCGFQKKTYSKMNNPAIKRI GNHITKSPEDKREYRGLELANGIKV LLMSDPTTDKSSAALDVHIGSLSDP PNIAGLSHFCEHMLFLGTKKYPKEN EYSQFLSEHAGSSNAFTSGEHTNYY FDVSHEHLEGALDRFAQFFLCPLFD ESCKDREVNAVDSEHEKNVMNDAWR LFQLEKATGNPKHPFSKFGTGNKYT LETRPNQEGIDVRQELLKFHSAYYS SNLMAVCVLGRESLDDLTNLVVKLF SEVENKNVPLPEFPEHPFQEEHLKQ LYKIVPIKDIRNLYVTFPIPDLQKY YKSNPGHYLGHLIGHEGPGSLLSEL KSKGWVNTLVGGQKEGARGFMFFII NVDLTEEGLLHVEDIILHMFQYIQK LRAEGPQEWVFQECKDLNAVAFRFK DKERPRGYTSKIAGILHYYPLEEVL TAEYLLEEFRPDLIEMVLDKLRPEN VRVAIVSKSFEGKTDRTEEWYGTQY KQEAIPDEVIKKWQNADLNGKFKLP TKNEFIPTNFEILPLEKEATPYPAL IKDTVMSKLWFKQDDKKKKPKACLN FEFFSPFAYVDPLHCNMAYLYLELL KDSLNEYAYAAELAGLSYDLQNTIY GMYLSVKGYNDKQPILLKKIIEKMA TFEIDEKRFEIIKEAYMRSLNNFRA EQPHQHAMYYLRLLMTEVAWTKDEL KEALDDVTLPRLKAFIPQLLSRLHI EALLHGNITKQAALGIMQMVEDTLI EHAHTKPLLPSQLVRYREVQLPDRG WFVYQQRNEVHNNCGIEIYYQTDMQ STSENMFLELFCQIISEPCFNTLRT KEQLGYIVFSGPRRANGIQSLRFII QSEKPPHYLESRVEAFLITMEKSIE DMTEEAFQKHIQALAIRRLDKPKKL SAECAKYWGEIISQQYNFDRDNTEV AYLKTLTKEDIIKFYKEMLAVDAPR RHKVSVHVLAREMDSCPVVGEFPCQ NDINLSQAPALPQPEVIQNMTEFKR GLPLFPLVKPHINFMAAKLTRTRPL EQKLISEEDLAANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
