Recombinant Human IgG1Fc protein

• Cat.No.: IgG1Fc-13H
• Product Overview: Recombinant Human IgG1Fc protein(P01857-1)(Asp104-Lys330) is expressed from HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Protein Length: Asp104-Lys330
• Tag: N/A
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Human FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Human IgG1 Fc, No Tag with an affinity constant of 0.28 μM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 25.5 kDa. Due to glycosylation, the protein migrates to 31-33 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
  > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt; -80°C for 3-6 months after reconstitution; 2-8°C for 2-7 days after reconstitution; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.