Recombinant Human IGHG2 protein
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|Product Overview :||Recombinant Human IGHG2 (Glu99-Lys326) protein carried no tag and was expressed in human 293 cells (HEK293).|
|Description :||Immunoglobulin G2 (IgG2) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. There are two members of IgG2: IgG2a and IgG2b. It was found that IgG2a was superior to IgG1 in activating complement. The glycosylation of the circulating immunoglobulin-γ (IgG) antibody molecules changes in rheumatoid arthritis. Ig gamma-2 chain Fc region contains two constant regions of IgG2 H chain (CH2, CH3).|
|Predicted N Terminal :||Glu99|
|Form :||Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 25.7 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Protein length :||Glu99-Lys326|
|Endotoxin :||Less than 1.0 EU per μg by the LAL method.|
|Purity :||>95% as determined by SDS-PAGE.|
|Storage :||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of IGHG2-548H :||
Human IgG Fc, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
|Bioactivity-SPR of IGHG2-548H :||
Human FCGRT&B2M Heterodimer Protein, His Tag (SPR & BLI verified) captured on CM5 Chip via anti-His antibody can bind Human IgG2 Fc, Tag Free (SPR verified) with an affinity constant of 33.3 nM as determined in SPR assay (Biacore 8K) (QC tested).
|Gene Name :||IGHG2|
|Official Symbol :||IGHG2|
|Synonyms :||IGHG2; Immunoglobulin heavy constant gamma 2(G2m marker)|
|Gene ID :||3501|
|UniProt ID :||P01859|