Recombinant Human IMPDH2, His-tagged

• Cat.No.: IMPDH2-28270TH
• Product Overview: Recombinant full length Human IMPDH2 with N-terminal His tag. 534 amino acids with a MWt 58kDa including tag.

Specification

• Description: This gene encodes the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. The encoded protein catalyzes the NAD-dependent oxidation of inosine-5-monophosphate into xanthine-5-monophosphate, which is then converted into guanosine-5-monophosphate. This gene is up-regulated in some neoplasms, suggesting it may play a role in malignant transformation.
• Protein length: 514 amino acids
• Conjugation: HIS
• Molecular Weight: 58.000kDa inclusive of tags
• Source: E. coli
• Tissue specificity: IMP type I is the main species in normal leukocytes and type II predominates over type I in the tumor.
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: pH: 8.00Constituents:0.32% Tris HCl, 0.03% DTT, 20% Glycerol, 0.88% Sodium chloride
• Storage: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHHSSGLVPRGSHMADYL ISGGTSYVPDDGLTAQQLFNCGDGL TYNDFLILPGYIDFTADQVDLTSAL TKKITLKTPLVSSPMDTVTEAGMAI AMALTGGIGFIHHNCTPEFQANEVR KVKKYEQGFITDPVVLSPKDRVRDV FEAKARHGFCGIPITDTGRMGSRLV GIISSRDIDFLKEEEHDCFLEEIMT KREDLVVAPAGITLKEANEILQRSK KGKLPIVNEDDELVAIIARTDLKKN RDYPLASKDAKKQLLCGAAIGTHED DKYRLDLLAQAGVDVVVLDSSQGNS IFQINMIKYIKDKYPNLQVIGGNVV TAAQAKNLIDAGVDALRVGMGSGSI CITQEVLACGRPQATAVYKVSEYAR RFGVPVIADGGIQNVGHIAKALALG ASTVMMGSLLAATTEAPGEYFFSDG IRLKKYRGMGSLDAMDKHLSSQNRY FSEADKIKVAQGVSGAVQDKGSIHK FVPYLIAGIQHSCQDIGAKSLTQVR AMMYSGELKFEKRTSSAQVEGGVHS LHSYEKRLF
• Sequence Similarities: Belongs to the IMPDH/GMPR family.Contains 2 CBS domains.

Gene Information

• Gene Name: IMPDH2 IMP (inosine 5-monophosphate) dehydrogenase 2 [ Homo sapiens ]
• Official Symbol: IMPDH2
• Synonyms: IMPDH2; IMP (inosine 5-monophosphate) dehydrogenase 2; inosine-5-monophosphate dehydrogenase 2
• Gene ID: 3615
• mRNA Refseq: NM_000884
• Protein Refseq: NP_000875
• Uniprot ID: P12268
• Chromosome Location: 3p21.2
• Pathway: Drug metabolism - other enzymes, organism-specific biosystem; Drug metabolism - other enzymes, conserved biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of nucleotides, organism-specific biosystem
• Function: DNA binding; IMP dehydrogenase activity; RNA binding; metal ion binding; nucleotide binding