Recombinant Human INCENP, His-tagged

• Cat.No.: INCENP-7037H
• Product Overview: Recombinant human INCENP (783-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. This product can be used for Aurora B and Aurora C activation in vitro.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Protein Length: 783 aa-end
• Description: INCENP or inner centromere protein antigens 135/155kDa is a constitutively binding centromere proteins and passenger, or transiently interacting, proteins. Theoverexpression of a truncation mutant of chicken INCENP and a chimeric CENPB:INCENP protein in mammalian cell culture disrupted both prometaphase chromosome alignment and completion of cytokinesis. INCENP forms a complex with the evolutionarily conserved family of Aurora B kinases which helps coordinate chromosome segregation, spindle behavior, and cytokinesis during mitosis.
• Form: Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
• Molecular Mass: ~19 kDa
• Purity: >90%
• Applications: Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: INCENP inner centromere protein antigens 135/155kDa [ Homo sapiens ]
• Official Symbol: INCENP
• Synonyms: INCENP; inner centromere protein antigens 135/155kDa; inner centromere protein antigens (135kD, 155kD); inner centromere protein; FLJ31633; chromosomal passenger protein; inner centromere protein INCENP; binds and activates aurora-B and -C in vivo and in vitro; MGC111393
• Gene ID: 3619
• mRNA Refseq: NM_001040694
• Protein Refseq: NP_001035784
• MIM: 604411
• UniProt ID: Q9NQS7
• Chromosome Location: 11q12.3
• Pathway: Aurora B signaling, organism-specific biosystem; Aurora C signaling, organism-specific biosystem; Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; DNA Replication, organism-specific biosystem; M Phase, organism-specific biosystem; Mitotic M-M/G1 phases, organism-specific biosystem
• Function: protein binding