Active Recombinant Human ITGAIIb & ITGB3 Heterodimer Protein, His-tagged

• Cat.No.: ITGAIIb&ITGB3-363H
• Product Overview: Recombinant Human ITGAIIb & ITGB3 Heterodimer Protein, His-tagged is expressed from human 293 cells (HEK293). It contains AA Leu 32 - Arg 993 (ITGAIIb) & Gly 27 - Asp 718 (ITGB3) (Accession # P08514-1 (R887L) (ITGAIIb) & P05106-1 (ITGB3)). Predicted N-terminus: Leu 32 (ITGAIIb) & Gly 27 (ITGB3). Human ITGAIIb & ITGB3 Heterodimer Protein, produced by co-expression of ITGAIIb and ITGB3, has a calculated MW of 111.4 kDa (ITGAIIb) & 81.8 kDa (ITGB3). Subunit ITGAIIb is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB3 contains no tag but a basic tail at the C-terminus. The reducing (R) protein migrates as 125 kDa and 145 kDa (ITGAIIb) and 110-120 kDa (ITGB3) respectively due to glycosylation.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Leu 32 - Arg 993 (ITGAIIb) & Gly 27 - Asp 718 (ITGB3)
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human Fibronectin at 2 μg/mL (100 μL/well) can bind Recombinant Human ITGAIIb & ITGB3 Heterodimer Protein, His-tagged with a linear range of 2-78 ng/mL (QC tested).
  Recombinant Human ITGAIIb & ITGB3 Heterodimer Protein, His-tagged at 1 μg/mL (100 μL/well) can bind Abciximab with a linear range of 0.8-6 ng/mL (Routinely tested).
• Molecular Mass: 111.4 kDa (ITGAIIb) & 81.8 kDa (ITGB3)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: ITGB3 integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) [ Homo sapiens ]
• Official Symbol: ITGB3
• Synonyms: ITGB3; GP3A; integrin beta-3; CD61; GPIIIa; platelet glycoprotein IIIa; GT; BDPLT2
• Gene ID: 3690
• mRNA Refseq: NM_000212
• Protein Refseq: NP_000203
• UniProt ID: P05106