Recombinant Human ITGAV&ITGB3 protein, His-Avi-tagged

• Cat.No.: ITGAV & ITGB3-311H
• Product Overview: Recombinant Human ITGAV&ITGB3 protein(P06756-1(ITGAV)&P05106-1(ITGB3))(Phe31-Val992(ITGAV)& Gly27-Asp718(ITGB3)), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Phe31-Val992(ITGAV)& Gly27-Asp718(ITGB3)
• Tag: C-His-Avi
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human ITGAV&ITGB3, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-alpha V beta3 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 114 kDa (ITGAV)&81.3 kDa (ITGB3). Due to glycosylation, the protein migrates to 85-140 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
• GeneID 2: 3690

Gene Information

• Gene Name: ITGAV integrin, alpha V [ Homo sapiens ]
• Official Symbol: ITGAV
• Synonyms: ITGAV; integrin, alpha V; antigen identified by monoclonal L230 , integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) , MSK8, vitronectin receptor , VNRA, VTNR; integrin alpha-V; CD51; integrin alphaVbeta3; vitronectin receptor subunit alpha; antigen identified by monoclonal L230; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); MSK8; VNRA; VTNR; DKFZp686A08142
• Gene ID: 3685
• mRNA Refseq: NM_001144999
• Protein Refseq: NP_001138471
• MIM: 193210
• UniProt ID: P06756
• Gene Name 2: ITGB3 integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) [ Homo sapiens ]
• Official Symbol 2: ITGB3
• Synonyms 2: ITGB3; integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61); GP3A; integrin beta-3; CD61; GPIIIa; platelet glycoprotein IIIa; platelet membrane glycoprotein IIIa; GT; BDPLT2
• mRNA Refseq 2: NM_000212
• Protein Refseq 2: NP_000203
• UniProt ID 2: P05106