Recombinant Human Josephin Domain Containing 1, His-tagged

• Cat.No.: JOSD1-7188H
• Product Overview: Recombinant human JOSD1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 25.6kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-202aa
• Description: JOSD1, also as known as Josephin-1, has low protease activity towards poly-ubiquitin chains (in vitro). JOSD1 is act as a deubiquitinating enzyme. Recently identified ubiquitin-binding sites in the Josephin domain contribute to ubiquitin chain binding and cleavage.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 5mM DTT, 2mM EDTA.
• Molecular Mass: 25.6kDa (225aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSCVPWK GDKAKSESLE LPQAAPPQIY HEKQRRELCA LHALNNVFQD SNAFTRDTLQ EIFQRLSPNT MVTPHKKSML GNGNYDVNVI MAALQTKGYE AVWWDKRRDV GVIALTNVMG FIMNLPSSLC WGPLKLPLKR QHWICVREVG GAYYNLDSKL KMPEWIGGES ELRKFLKHHL RGKNCELLLV VPEEVEAHQS WRTDV
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: JOSD1 Josephin domain containing 1 [ Homo sapiens ]
• Official Symbol: JOSD1
• Synonyms: JOSD1; Josephin domain containing 1; Josephin-1; KIAA0063; josephin domain-containing 1; dJ508I15.2
• Gene ID: 9929
• mRNA Refseq: NM_014876
• Protein Refseq: NP_055691
• UniProt ID: Q15040
• Chromosome Location: 22q13.1
• Function: omega peptidase activity; peptidase activity