Recombinant Human KAZALD1 protein, His & T7-tagged

• Cat.No.: KAZALD1-1872H
• Product Overview: Recombinant Human KAZALD1 aa. (Arg31~Tyr304 (Accession # Q96I82)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Arg31~Tyr304
• Description: This gene encodes a secreted member of the insulin growth factor-binding protein (IGFBP) superfamily. The protein contains an insulin growth factor-binding domain in its N-terminal region, a Kazal-type serine protease inhibitor and follistatin-like domain in its central region, and an immunoglobulin-like domain in its C-terminal region. Studies of the mouse ortholog suggest that this protein may function in bone development and bone regeneration. This gene is hypomethylated and over-expressed in high-grade glioma compared to low-grade glioma, and thus the hypomethylated gene may be associated with cell proliferation and the shorter survival of patients with high-grade glioma. It is also one of numerous genes found to be deleted in a novel 5.54 Mb interstitial deletion, which is associated with multiple congenital anomalies. Alternative splicing results in multiple transcript variants.
• Form: Freeze-dried powder
• Molecular Mass: 37kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >90%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 4.6

Gene Information

• Gene Name: KAZALD1 Kazal type serine peptidase inhibitor domain 1 [ Homo sapiens (human) ]
• Official Symbol: KAZALD1
• Synonyms: KAZALD1; Kazal type serine peptidase inhibitor domain 1; BONO1; FKSG28; FKSG40; IGFBP-rP10; kazal-type serine protease inhibitor domain-containing protein 1; IGFBP-related protein 10; bone- and odontoblast-expressed gene 1; novel kazal-type serine protease inhibitor domain and immunoglobulin domain containing protein
• Gene ID: 81621
• mRNA Refseq: NM_001319303.1
• Protein Refseq: NP_001306232.1
• UniProt ID: Q96I82