Recombinant Human KDR, Fc-His

• Cat.No.: KDR-1697H
• Product Overview: Recombinant Human VEGFR2 encoding amino acid residues 1 - 764 of the extracellular domain of human VEGFR2 was fused to a 6X His-tagged Fc of human IgG1 via the peptide (IEGRMD). It was expressed inNSO Cells.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: Fc&His
• Description: Vascular endothelial growth factor receptor 2 (VEGF R2) is a transmembrane glycoprotein that is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration, and differentiation.
• State Of Matter: Lyophilized.
• Purity: >90% by SDS Page and analyzed by silver stain.
• Endotoxin: <1.0 EU/µg as determined by the LAL method.
• Molecular Weight: The predicted molecular weight of Recombinant Human KDR is Mr 110 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 160-170 kDa.
• Biological Activity: The biological activity of Human KDR was determined by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells. The expected ED50 for this effect is typically 4-8 ng/ml in the presence of 5 ng/ml recombinant human VEGF165.
• Storage And Stability: This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Gene Information

• Gene Name: KDR kinase insert domain receptor (a type III receptor tyrosine kinase) [ Homo sapiens ]
• Synonyms: KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); FLK1; CD309; VEGFR; VEGFR2; Vascular endothelial growth factor receptor 2; VEGFR-2; Kinase insert domain receptor; Protein-tyrosine kinase receptor Flk-1; EC 2.7.10.1; fetal liver kinase-1; soluble VEGFR2; tyrosine kinase growth factor receptor
• Gene ID: 3791
• mRNA Refseq: NM_002253
• Protein Refseq: NP_002244
• MIM: 191306
• UniProt ID: P35968
• Chromosome Location: 4q11-q12
• Pathway: Cytokine-cytokine receptor interaction; Endocytosis; Focal adhesion; VEGF signaling pathway; Signaling by VEGF
• Function: ATP binding; growth factor binding; nucleotide binding; receptor activity; transferase activity; vascular endothelial growth factor receptor activity