Recombinant Human KEAP1 Protein (1-624 aa), His-tagged

• Cat.No.: KEAP1-2185H
• Product Overview: Recombinant Human KEAP1 Protein (1-624 aa) is produced by Yeast expression system. This protein is fused with a 6xHis tag at the N-terminal. Research Area: Epigenetics and Nuclear Signaling. Protein Description: Full Length.

Specification

• Description: Acts as a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1 and targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. Retains NFE2L2/NRF2 and may also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.
• Source: Yeast
• Species: Human
• Tag: His
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 71.7 kDa
• Protein length: 1-624 aa
• AA Sequence: MQPDPRPSGAGACCRFLPLQSQCPE GAGDAVMYASTECKAEVTPSQHGNR TFSYTLEDHTKQAFGIMNELRLSQQ LCDVTLQVKYQDAPAAQFMAHKVVL ASSSPVFKAMFTNGLREQGMEVVSI EGIHPKVMERLIEFAYTASISMGEK CVLHVMNGAVMYQIDSVVRACSDFL VQQLDPSNAIGIANFAEQIGCVELH QRAREYIYMHFGEVAKQEEFFNLSH CQLVTLISRDDLNVRCESEVFHACI NWVKYDCEQRRFYVQALLRAVRCHS LTPNFLQMQLQKCEILQSDSRCKDY LVKIFEELTLHKPTQVMPCRAPKVG RLIYTAGGYFRQSLSYLEAYNPSDG TWLRLADLQVPRSGLAGCVVGGLLY AVGGRNNSPDGNTDSSALDCYNPMT NQWSPCAPMSVPRNRIGVGVIDGHI YAVGGSHGCIHHNSVERYEPERDEW HLVAPMLTRRIGVGVAVLNRLLYAV GGFDGTNRLNSAECYYPERNEWRMI TAMNTIRSGAGVCVLHNCIYAAGGY DGQDQLNSVERYDVETETWTFVAPM KHRRSALGITVHQGRIYVLGGYDGH TFLDSVECYDPDTDTWSEVTRMTSG RSGVGVAVTMEPCRKQIDQQNCTC
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA will be sent along with the products. Please refer to it for detailed information.

Gene Information

• Gene Name: KEAP1 kelch-like ECH-associated protein 1 [ Homo sapiens ]
• Official Symbol: KEAP1
• Synonyms: KEAP1; INrf2; KIAA0132; KLHL19; MGC1114; MGC4407; MGC9454; MGC10630; MGC20887
• Gene ID: 9817
• mRNA Refseq: NM_012289
• Protein Refseq: NP_036421
• MIM: 606016
• UniProt ID: Q14145