Active Recombinant Human KLK11 Protein, His-tagged, Low Endotoxin

• Cat.No.: KLK11-075H
• Product Overview: Active Recombinant Human KLK11 Protein(Q9UBX7-2)(Ile54-Asn282) is expressed from HEK293 with His tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 54-282 aa
• Form: Lyophilized from 0.22 μm filtered solution in 20mM HEPES, 150mM NaCl, 8% trehalose, 0.05% Brij-35 (pH 7.5).
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate: N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is > 1200pmol/min/µg.
• Molecular Mass: The protein has a predicted MW of 26.85 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: KLK11 kallikrein-related peptidase 11 [ Homo sapiens ]
• Official Symbol: KLK11
• Synonyms: KLK11; kallikrein-related peptidase 11; kallikrein 11 , PRSS20; kallikrein-11; TLSP; hK11; hippostasin; kallikrein 11; serine protease 20; trypsin-like protease; PRSS20; MGC33060
• Gene ID: 11012
• mRNA Refseq: NM_001136032
• Protein Refseq: NP_001129504
• MIM: 604434
• UniProt ID: Q9UBX7