Active Recombinant Human KLRK1 protein, His-Avi-tagged, Biotinylated, Low Endotoxin

• Cat.No.: KLRK1-718HB
• Product Overview: Biotinylated Active Recombinant Human KLRK1 protein(P26718)(Phe78-Val216) is expressed from HEK293 with Avi tag and His tag at the N-Terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 78-216 aa
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Biotinylated Human NKG2D, His-Avi Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human MICA, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 19.0 kDa. Due to glycosylation, the protein migrates to 36-40 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: KLRK1 killer cell lectin-like receptor subfamily K, member 1 [ Homo sapiens ]
• Official Symbol: KLRK1
• Synonyms: KLRK1; killer cell lectin-like receptor subfamily K, member 1; D12S2489E, DNA segment on chromosome 12 (unique) 2489 expressed sequence; NKG2-D type II integral membrane protein; CD314; KLR; NKG2 D; NKG2D; NK cell receptor D; NKG2-D-activating NK receptor; NKG2-D; D12S2489E; FLJ17759; FLJ75772
• Gene ID: 22914
• mRNA Refseq: NM_007360
• Protein Refseq: NP_031386
• MIM: 611817
• UniProt ID: P26718