Recombinant Human LAYN protein(Met1-Glu220), hFc-tagged

• Cat.No.: LAYN-78H
• Product Overview: Recombinant Human LAYN(NP_849156.1)(Met 1-Glu 220) was expressed in HEK293 with the C-terminal fused Fc region of Human IgG1.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 1-220 a.a.
• Form: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human LAYN/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 437 amino acids and predicts a molecular mass of 49.5 kDa. As a result of glycosylation, the rhLAYN/Fc monomer migrates as an approximately 60-65 kDa protein in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: LAYN layilin [ Homo sapiens ]
• Official Symbol: LAYN
• Synonyms: LAYN; layilin; FLJ30977; FLJ31092
• Gene ID: 143903
• mRNA Refseq: NM_178834
• Protein Refseq: NP_849156
• UniProt ID: Q6UX15