Active Recombinant Human LCAT protein, His-tagged

• Cat.No.: LCAT-526H
• Product Overview: Active Recombinant Human LCAT protein(NP_000220.1) (Phe25-Glu440) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 25-440 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Immobilized Human LCAT, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-LCAT Antibody, hFc Tag with the EC50 of 9.0ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 48.2 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: LCAT lecithin-cholesterol acyltransferase [ Homo sapiens ]
• Official Symbol: LCAT
• Synonyms: LCAT; lecithin-cholesterol acyltransferase; phosphatidylcholine-sterol acyltransferase; phospholipid-cholesterol acyltransferase
• Gene ID: 3931
• mRNA Refseq: NM_000229
• Protein Refseq: NP_000220
• MIM: 606967
• UniProt ID: P04180