Recombinant Human LIPG Protein, His-tagged

• Cat.No.: LIPG-45H
• Product Overview: Recombinant Human LIPG(Ser21–Pro500) fused with N-terminal linker (2 extra AA), C-terminal linker (2 extra AA) and C-terminal His-tag (6 extra AA) was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Ser21–Pro500
• Description: The protein encoded by this gene has substantial phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. This protein is designated a member of the TG lipase family by its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family.
• Form: Filtered (0.4 μm) and lyophilized in phosphate buffered saline pH=7.5 (PBS), 1% (w/v) Sucrose, 4% (w/v) Mannitol
• Molecular Mass: 55.8 kDa (calculated)
• AA Sequence: ASSPVPFGPEGRLEDKLHKPKATQTEVKPSVRFNLRTSKDPEHEGCYLSVGHSQPLEDCSFNMTAKTFFIIHGWTMSGIFENWLHKLVSALHTREKDANVVVVDWLPLAHQLYTDAVNNTRVVGHSIARMLDWLQEKDDFSLGNVHLIGYSLGAHVAGYAGNFVKGTVGRITGLDPAGPMFEGADIHKRLSPDDADFVDVLHTYTRSFGLSIGIQMPVGHIDIYPNGGDFQPGCGLNDVLGSIAYGTITEVVKCEHERAVHLFVDSLVNQDKPSFAFQCTDSNRFKKGICLSCRKNRCNSIGYNAKKMRNKRNSKMYLKTRAGMPFRVYHYQMKIHVFSYKNMGEIEPTFYVTLYGTNADSQTLPLEIVERIEQNATNTFLVYTEEDLGDLLKIQLTWEGASQSWYNLWKEFRSYLSQPRNPGRELNIRRIRVKSGETQRKLTFCTEDPENTSISPGRELWFRKCRDGWRMKNETSPTVELPKLHHHHHH
• Endotoxin: < 1.0 EU/ug
• Purity: Purity as determined by densitometric image analysis: > 85 %
• Applications: ELISA, Cell culture and/or animal studies
• Storage: Store the lyophilized protein at –80 centigrade. Lyophilized protein remains stable until the expiry date when stored at –80 centigrade. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 centigrade for long term storage. Reconstituted protein can be stored at 4 centigrade for a week.
• Reconstitution: Add 200 µl of deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.

Gene Information

• Gene Name: LIPG lipase, endothelial [ Homo sapiens ]
• Official Symbol: LIPG
• Synonyms: LIPG; lipase, endothelial; endothelial lipase; EDL; lipoprotein lipase H; endothelial cell-derived lipase; EL; PRO719
• Gene ID: 9388
• mRNA Refseq: NM_006033
• Protein Refseq: NP_006024
• MIM: 603684
• UniProt ID: Q9Y5X9